Sericulture is an important agro-based livelihood activity in Assam, where Muga and Eri culture sustain rural economies while preserving cultural traditions. Tribal farm women are central to this sector, contributing to host plant management, silkworm rearing, and cocoon processing. Despite their active involvement, knowledge gaps in scientific practices often restrict productivity. The present study aimed to assess the knowledge level of tribal farm women engaged in Muga and Eri culture in Dhemaji district of Assam. A purposive-cum-random sampling method was adopted, wherein 120 respondents were selected from 10 villages. Data were collected through personal interviews using a structured knowledge test comprising 40 items—20 each on Muga and Eri. Scores were categorized into low, medium, and high knowledge groups using mean and standard deviation. The results revealed that 63.34% of respondents had medium knowledge, 22.50% high knowledge, and 14.16% low knowledge. Awareness was strong regarding host plants and general rearing practices, but limited in technical aspects such as pest and disease management and cocoon processing. The findings highlight the need for targeted training, practical demonstrations, and stronger extension support. Bridging these knowledge gaps will enhance productivity, promote adoption of improved practices, and ensure sustainable sericulture-based livelihoods in tribal communities.