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P-ISSN: 2618-060X, E-ISSN: 2618-0618   |   Impact Factor: RJIF 5.24, NAAS (2024): 5.20

2024, Vol. 7, Special Issue 7

Determination of LD50, germination percentage and seedling survivability for induced mutagenesis through gamma rays in French marigold (Tagetes patula L.)


A Ranjitha Ayodhya, R Vasantha Kumari and M Shalini

Marigold known as loose flower crop belongs to Asteraceae family. The impact of gamma radiations in developing new marigold mutants was carried out at Department of Horticulture, University of Agricultural Sciences, GKVK, Bengaluru. Seeds of French marigold var Arka madhu were collected and irradiated with various dosages viz. 50, 100, 150, 200, 250 and 300 Grays of gamma rays and control (untreated) to induce mutation. Seeds were sown in the nursery bed just after irradiation. LD50, seed germination percent and seed survivability percent was determined. LD50 was determined on survival basis. LD50 it is a dose that causes 50 percent mortality to the seeds. LD50 gives an indication of the response on different types of explants to mutagen so that the right dose to obtain beneficial mutants with minimal population loss. All the untreated control has shown 100 per survivability, but in case of gamma irradiated seeds, survivability was decreasing with increasing dosage of gamma ray. Thus, 50 percent survivability was observed in 150 Gy (49.46). The maximum germination, 93 percent was seen in untreated seeds i.e control followed by 85 percent in treatment comprising of 50 Gy gamma irradiation with 8 percent reduction in germination over the control. The maximum survival percentage was recorded in untreated seeds control group (97.47%) followed by 87.58 percent in treatment comprising of 50 Gy gamma irradiation. It was concluded that as the dosage increases, seed germination percent and survivability percent decreased.
Pages : 711-714 | 160 Views | 72 Downloads
How to cite this article:
A Ranjitha Ayodhya, R Vasantha Kumari, M Shalini. Determination of LD50, germination percentage and seedling survivability for induced mutagenesis through gamma rays in French marigold (Tagetes patula L.). Int J Res Agron 2024;7(7S):711-714. DOI: 10.33545/2618060X.2024.v7.i7Sj.1181
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