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P-ISSN: 2618-060X, E-ISSN: 2618-0618   |   Impact Factor: RJIF 5.24, NAAS (2024): 5.20

2024, Vol. 7, Special Issue 5

Effect of half-strength MS medium supplemented with plant growth regulators on in vitro response in ‘Bird of Paradise’ (Strelitzia reginae L.)


S Swagath Kumar, Anuradha Sane, M Lakshminarayana Reddy, Leela Sahijram and AVD Dorajee Rao

Propagation of Bird of Paradise (Strelitzia reginae L.) from seeds is a slow and complicated process due to the hard endocarp that encloses the seed and hinders germination. A study was undertaken to optimize in vitro protocol for mass multiplication of ‘Bird of Paradise’ (Strelitzia reginae L.). The experiment consisted of four treatments with growth regulators, viz., GA3 @1mg L-1; GA3 @3mg L-1; GA3 @5mg L-1; and, GA3 @1mg L-1 + BA @1mg L-1 with various medium components (½ MS + Sucrose @1% + Gelrite 3g L-1 + Inositol 100mg L-1 + Thiamine-HCl @ 1mg L-1 + Biotin 2mg L-1; and, medium with ½ MS + Sucrose @1% + Gelrite 3g L-1 + Inositol 100mg L-1 + Thiamine- HCl @1mg L-1 + Biotin 2mg L-1 (Control). Results from the experiment showed the significant differences between the treatments. The maximum rate of contamination was found in control while, minimum was in GA3 @ 5 mg L-1 and GA3 @ 1mg L-1+ BA @ 1 mg L-1 treatments. Higher rate and percentage of culture response (zygotic embryos germinated) were recorded in GA3 @ 1 mg L-1. Growth parameters such as shoot length (cm), root length (cm) and number of leaves per plant were maximum in ½ MS+ Sucrose @ 1% + Gelrite 3 g L-1 + Inositol 100 mg L-1 + Thiamine- HCl @ 1 mg L-1 + Biotin 2 mg L-1 + GA3 @ 1 mg L-1+BA @ 1 mg L-1 treatments. On the basis of overall results, our study confirmed that the media composed of ½ MS+ Sucrose @ 1% + Gelrite 3g L-1 + Inositol 100mg L-1+ Thiamine-HCl @1mg L-1+ Biotin 2mg L-1+ GA3 @1mg L-1 accelerated the germination rate and the combination of GA3 @1mg L-1 + BA @1mg L-1 growth regulators showed the better growth performance in in vitro propagation of ‘Bird of Paradises’ (Strelitzia reginae L.).
Pages : 116-120 | 228 Views | 111 Downloads
How to cite this article:
S Swagath Kumar, Anuradha Sane, M Lakshminarayana Reddy, Leela Sahijram, AVD Dorajee Rao. Effect of half-strength MS medium supplemented with plant growth regulators on in vitro response in ‘Bird of Paradise’ (Strelitzia reginae L.). Int J Res Agron 2024;7(5S):116-120. DOI: 10.33545/2618060X.2024.v7.i5Sb.722
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