Comparative genomic analysis for phylogeny, physio-chemical and anti-microbial properties in unconventional dairy animals
Ayushi Singh, Ravina, Chandana Sree Chinnareddyvari, Dharamshaw CA, Pallavi Rathi, Rangasai Chandra Goli and Kiyevi G Chishi
Casein is a major milk protein that affects the chemical and anti-microbial properties of milk. Four casein proteins i.e. alphaS1, alphaS2, beta, and kappa— were computed for the construction of a phylogenetic tree. Moreover, computational analysis was also performed for the physio-chemical and anti-microbial properties of milk. Whole protein sequences were retrieved from the NCBI database. Physiochemical properties and anti-microbial peptides were predicted using the ProtParam tool and AMPA Tool, respectively. Phylogenetic analysis revealed strong relatedness between Camel and Yak as compared to Donkey. Moreover, most of the casein proteins were found to be unstable in the instability index. Few bactericidal stretches were found especially alphaS2 casein protein in Camel, Yak and Donkey. The average anti-microbial index was around 0.25 -0.26. This study revealed about phylogenetic relatedness of casein protein in different species. It also highlighted the importance of the bioinformatics approach for better prediction of physio-chemical and anti-microbial features of casein micelles.
