2024, Vol. 7, Special Issue 10

author(s)
Munnesh Kumar, Kapil Kumar Nagar, Sharad V Pawar, B Pawar and DA Gadekar

abstract
A total of 37 alleles were amplified using five ISSR primers, with an average of 7.4 polymorphic bands per primer. The number of alleles per locus ranged from 5 to 12, while the polymorphism information content (PIC) values varied between 0.675 and 0.868. ISSR primers demonstrated strong performance in detecting genetic diversity in wheat and are recommended for future wheat improvement programs. Genomic DNA was extracted from 33 wheat genotypes using the CTAB method and subsequently amplified with the ISSR primers. The similarity matrix of the genotypes was calculated using NTSYSpc 2.1 software, and clustering was performed using the UPGMA (Unweighted Pair Group Method with Arithmetic Mean) method. The primary objective of this study was to assess genetic polymorphism and diversity, select promising diverse progenitors, and identify putative and informative markers that could be valuable for future crop improvement initiatives.