Mutagenic effectiveness and efficiency in M2 generation of foxtail millet (Setaria italica (L.) Beauv)

An experiment entitled “Mutagenic effectiveness and efficiency in M₂ generation of Foxtail millet (Setaria italica (L.) Beauve)” was carried out during Kharif 2024-25 at the Department of Agriculture Botany, VNMKV, Parbhani. The experiment was laid out in Randomized Block Design (RBD) with 11 Genotype and three replications to estimate the frequency and spectrum of chlorophyll and viable mutation in M₂ generation. For this purpose, seeds of Foxtail millet Siya 3156 seeds were treated with different doses of Gamma rays ranging from 100Gy, 200Gy, 300Gy, varying concentration of ethyl methane sulphonate (EMS) 0.1, 0.2, 0.3 and 0.4 and their combination of Gamma and EMS i.e 100Gy+0.1EMS, 100Gy+0.2EMS and 200Gy+0.1EMS. found of chlorophyll mutants and viable mutants like Xantha, Chlorina, Viridis, Xanthaviridis and viable mutants like Long, Loose, Compact panicle, bold Seeded panicle, pigmented glume panicle, Dwarf panicle. Among the all treatment mutagenic frequency and effectiveness maximum found in 100Gy+0.1 EMS followed by 200Gy+0.1EMS. Among all 12 characters like Days to 50% flowering, plant height, No. of productive tillers per plant, No. of tillers per plant, Internodal distance, Peduncle length, No. of ears per plant, Flag leaf area, Ear length, Grain yield per ear head, Grain yield per plant, 1000 grain weight.