2024, Vol. 7, Issue 3, Part H

Linseed (Linum usitatissimum L.) is a vital oilseed and fiber crop grown for its seed still as fiber which is employed for the manufacture of linen. The oil is edible and even has industrial use like preparation of paints, varnishes, printing ink, soap, oil, cloth, leather, and waterproof fabrics. In the present study the investigation done on linseed morphology and molecular screening for drought tolerance. Under normal conditions among all genotypes earliest flowering was observed in DEEPIKA (41 days) and under stress condition KARTIKA (41 days) and DEEPIKA (41 Days). Under normal conditions among all lines, the tallest plant was observed in normal condition is RLC-172(55.80 cm) and under stress condition is RLC-172 (56.10 cm). Under normal conditions the maximum number of fertile capsules observed in RLC-161(24) and under stress condition in RLC-143 and RLC- 92(15). The maximum 1000 seed weight observed under normal conditions in RLC- 167(6.80 gm) and under stressed conditions in RLC-172 (6.15 gm). The stressed linseed genotypes showed rise in proline accumulation over unstressed control. Under normal conditions the highest proline content observed in KIRAN (6.62 μmol/g) and under stress condition in KIRAN (18.89 μmol/g). The maximum root length observed in normal condition is KARTIKA (8.6 cm) and under stressed condition observed in KARTIKA (8.96 cm).

The present ISSR primers investigation revealed that the mean polymorphic information content (PIC) score for each primer ranged from 0.34 to 0.71 with a mean of 0.55. The least relevant primer was noticed to be ISSR primer UBC-807 of PIC value of 0.34, while the maximum informative marker UBC-825 had a PIC value of 0.71. The similarity matrix of 18 linseed lines varies from 0.20(IA-32-DEEPIKA) to 0.92(RLC-167-RLC-148). Thus, most similar genotypes observed were RLC-167 and RLC-148. The most dissimilar genotypes observed were IA-32 and DEEPIKA were noticed. The dissimilarity matrix of 18 linseed lines varies from 0.08(RLC-167- RLC-148) to 0.77 (IA-32-DEEPIKA). Thus, most dissimilar genotypes observed were IA-32-DEEPIKA. The most dissimilar genotypes observed were RLC-167-RLC-148 were noticed. The present SSR primers investigation revealed that the mean polymorphic information content (PIC) score for each primer ranged from 0.15 to 0.84. The least relevant primer was noticed to be SSR primer LU-9 of PIC value of 0.15, while the maximum informative marker LU-3 had a PIC value of 0.84. Thus, most similar genotypes observed were RLC-92 and RLC-153 and RLC-133. The most dissimilar genotypes observed were IA-32 and KIRAN were noticed. The dissimilarity matrix of 18 linseed lines varies from 0.00(RLC-92-RLC-153) to 0.77 (IA-32-KIRAN). Thus, most dissimilar genotypes observed were IA-32-KIRAN. The most dissimilar genotypes observed were RLC-92 and RLC-153 were noticed.