2024, Vol. 7, Issue 10, Part F

author(s)
Jyothi Veerayya Hosamath, Ramakrishna V Hegde, Yashaswini Sharma, BN Aravindkumar and Umesh V Mummigatti

abstract
The experiment on “In vitro screening of coriander genotypes for moisture stress tolerance using polyethylene glycol-6000 (PEG-6000)” was carried out at the Department of Horticulture, College of Agriculture, University of Agricultural Sciences, Dharwad, Karnataka during the year 2021-2022. The local genotype DWD-3 was evaluated for germination of seeds for drought stress using different concentrations of PEG-6000 at 0, 5, 10, 15, 20 and 25%. The germination percentage decreased as the concentration of PEG-6000 increased. Further, the probit analysis was done to determine LD₅₀ value of PEG-6000 for coriander and it was observed as 12.70%. PEG-6000 at two concentrations derived from the standardization studies (12.70% as LD₅₀ dose and another 10% as just below the LD₅₀) along with control (0%) were imposed for screening the available twenty genotypes. Results indicated that seedling parameters of coriander differed significantly with respect to different stress levels, genotypes and their interactions. Seed germination, shoot length and seedling vigour index were decreased under stress conditions imposed by increased PEG concentrations from 0 to 10 and 12.70%. On the contrary, the root length increased under 10% PEG as compared to non-stress condition and decreased further at 12.70%. Whereas, root to shoot ratio increased linearly as the PEG concentration increased. The shoot length stress tolerance index and root length stress tolerance index were found to be the highest at 10% PEG and declined later as the concentration increased to 12.70%. Finally, eight genotypes viz., RCr-728, RCr-41, ACr-2, RCr-446, RCr-20, RCr-435, CO-3 and ACr-1 were identified as drought tolerant.