2024, Vol. 7, Issue 1, Part D

Sorghum (Sorghum bicolor L.) is a cereal plant that can support food in Indonesia because it contains sufficient nutrients. In addition, sorghum has potential as a vegetable herbicide because it has allelochemical compounds of phenolic groups such as cyanogenic (Dhurin) and sorgoleone, which can inhibit weed growth. This study aimed to obtain the optimum concentration of sorghum allelochemicals on sorghum's germination and vegetative growth inhibition and determine the Inhibitory Concentration (IC50). The research was carried out in the Agronomy Laboratory and the greenhouse of the Faculty of Agriculture, Bengkulu University, Indonesia. The design used in this study was a completely randomized design (CRD) with a single factor. The factors tested were the herbicide concentration of sorghum (C), namely C₁ = 0%, C₂ = 2.5%, C₃ = 5%, C₄ = 7.5%, and C₅ = 10%. Each treatment was repeated five times so that 25 experimental units were obtained. Each experimental unit consists of two petrifies and two polybags. The data obtained were analyzed statistically using Analysis of Variance (ANOVA) at a significance level of F 5%. Variables that had a significant effect were further tested for Orthogonal Polynomials, and to determine the IC50, the data obtained were analyzed using regression analysis. The study's results showed sorghum bioherbicide inhibits seedling growth as evidenced by short primary roots, malformed sprouts, twisted plumules, swelling and short cotyledons, a small number of leaves, and stunting at concentrations ranging from 2.5% to 10%. The IC50 value of in vitro sorghum bioherbicide is 5.85 for the normal seedling percentage.